Coding

Part:BBa_K5335025:Design

Designed by: Yuanpu Zhang   Group: iGEM24_HZAU-China   (2024-09-29)


SpyCatcher-VDAL-CPPs (R9-Tag)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When using both R9 cell-penetrating peptide and Spycatcher tags, we employ a flexible protein linker to connect them to our VDAL protein. This is to prevent steric hindrance from affecting the activity of our core protein. Additionally, it's crucial to pay attention to the orientation of these protein tags, as they both have C-terminal and N-terminal activities.


Source

VDAL is a protein from the Verticillium dahliae.


References